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de Matos DG, Furnus CC, Moses DF: Glutathione synthesis during in vitro maturation of bovine oocytes: role of cumulus cells

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doi: 10.1016/j.cub.2014.03.034 9 MassartCHosteCVirionARufJDumontJEVan SandeJ

Each tardigrade was sonicated for 90 s in rearing water to allow the entrance of the probe in the tardigrade, and then incubated in 1 ml of 10 M DCFH 2 -DA in methanol for 45 min at 20 C in the dark
[PubMed: 7868816] 460