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2009;120(7):13461351 [DOI] [PubMed] [Google Scholar] 55

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The study used databases at the Toronto-based Institute for Clinical Evaluative Sciences to review the patient records of 146,850 residents of the province aged 65 or over who were prescribed regular vitamin B12 shot between January 2011 and October 2015

The latter involves dissociation of IGFBPs from IGF-1 by low pH, followed by ethanol precipitation and removal via centrifugation