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Levocarnitine can be metabolized to trimethylamine, an amine with a distinctive fish-like odor that is excreted in sweat, breath, and urine

The resultant amplicons were digested with 2 U BslI (New England Biolabs Japan, Tokyo, Japan) for 1 h and then fractionated using an automated sequence analyzer (ABI PRISM 3130xl Genetic Analyzer, Applied Biosystems, Foster City, CA, USA)

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