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Whatever your protein source is, youll want to load it onto the column in pretty much the same way cap the bottom, add your interacting protein or cell lysate, cap the top, and incubate with gentle agitation for around an hour

We used a microarray data set from the public database library of GEO (Gene Expression Omnibus), which is associated with liver transcriptome in the early stages of copper accumulation in the mouse model of Wilsons disease under Atp7b knockout

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