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Notably, several genes involved in glycolysis/gluconeogenesis (GNG) pathways, such as alcohol dehydrogenase 7 ( Adh7 ) and glucokinase ( Gck ), and genes related to antioxidant defense, including gamma-glutamyltransferase 6 ( Ggt6 ), glutathione S-transferase pi 3 ( Gstp3 ), and microsomal glutathione S-transferase 2 ( Mgst2 ), were downregulated in response to SIRT1 deletion (Fig

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Peptides were eluted twice in 200 L of 10% (v/v) formic acid for 5 min into a 96-deep well plate