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Cellular assays Cell lines For generating clonal ZSWIM8 -knockout K562s, parental K562 cells harbouring a miR-7-sensitive GFP reporter 10 were transiently transfected with a plasmid derived from PX458 (Addgene, 48138) expressing pSpCas9-2a-BFP and either one or two single guide RNAs (sgRNAs) targeting the ZSWIM8 gene (Supplementary Table 4), followed by single-cell sorting of BFP-positive cells

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Botox is said to be the most effective treatment for this type of facial paralysis