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DNA organisation at three levels may be observed after sperm chromatin protamination: (1) toroidal structures shaped by protamines (9095 %), (2) nucleosomes (5 %10 %) taking part in the chief stages of the development of embryo alongside (3) the so-called matrix binding sites

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4 out of 10 genes encode lysosomal lipases including lipl-1 , lipl-3 , lipl-4 , and lipl-7 , while the other 6 genes are involved in sphingolipid metabolism and proteolysis, including asah-1 , asm-2 , and asm-3 for sphingolipid metabolism, and cpr-1, cpr-4, and cpr-5 for proteolysis (Fig

J., Johnson, T

For qRT-PCR experiments mRNA was extracted from bleach synchronized worms by Tri-reagent (Catalog Number T9424, Sigma-Aldrich Canada) according to the manufacturers instructions