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After incubation at 37C for 2 h, 100 l of the supernatant was collected for ultrahigh performance liquid chromatography-electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) analysis of the specific metabolites of the six CYP variants: acetaminophen (CYP1A), OH-bupropion (CYP2B6), 4-OH-mephenytoin (CYP2C19), 4-OH-diclofenac (CYP2C9), OH-bufuralol (CYP2D6) and 1-OH-midazolam (CYP3A)

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