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This includes meticulous surgical technique, optimized pain management protocols, early mobilization, and targeted nutritional support

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The membranes were then washed several times with water and TBS (PBS, 0.05% Tween-20), a 1 :5000 dilution of horseradish-peroxidase conjugated goat anti-rabbit antiserum was added, and the membranes further incubated for 1 h at room temperature

Thank you again to all who participated - your support means so much