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To investigate this, we generated lysine-to-arginine (KR) and lysine-to-tryptophan (KW) HMGB1/2 mutants, which prevent or mimic K-Hcy, respectively
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Following reagents were used: ethylenediaminetetraacetic acid (EDTA, 99.995% trace metal basis) and diethylenetriaminepentaacetic acid (DTPA) from Sigma/Merck (Merck KGaA, Darmstadt, Germany), nitrilotriacetic acid (NTA) and L-amino acids (His, Glu and Gly) from Fluka (Merck KGaA, Darmstadt, Germany)
Unfortunately, research investigating the simultaneous experimentation with all three compounds has yet to be conducted
The clinical sequelae of intravascular hemolysis and extracellular plasma hemoglobin: a novel mechanism of human disease
S.SharmaG.ChowdhuryJ
