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Likewise, the two consecutive serine residues (Ser462/Ser463 in EcGGT) of small subunit are replaced by histidine and threonine (414His/415Thr in GtGGT) in GGTs from extremophiles

Hanikoglu A, Ozben H, Hanikoglu F, Ozben T

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However, studies that have investigated GABA B binding in the frontal cortex 61,62 and in the temporal cortex and hippocampus 62 found no evidence of altered GABA B -receptor binding, with the exception of an increased affinity in the temporal cortex of drug-free suicide victims

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An advantage of on-column cleavage is the high level of purity obtained, but this comes at the expense of generally low yield due to less efficient protease cleavage and decreased control of the digestion conditions