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10.3389/fnbeh.2014.00054 2 AgrawalV.SmelserD

If a second primary antibody was used, the cells were incubated either for 2 h at room temperature or overnight at 4C

However, certain nutrients can help protect existing egg quality, reduce oxidative stress and optimise the eggs that are still available, which may improve response to natural cycles or fertility treatments
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doi: 10.1016/j.arr.2016.08.002