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In addition, partial overexpression of histone H3 and H4 reversed the transcription defects observed during ageing and reduced the reverse transcriptional transposition, which indicated that the increased reverse transcriptional transposition in old yeast was the result of histone loss during ageing

An aliquot (50 L) of peanut samples (equivalent to a final assay concentration of 1 mg/mL) or buffer (blank) was first added to a clear, flat bottom 96-well plate followed by 50 L of 1,10-phenanthroline and then 50 L of FeSO4

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