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Half of the cells were pretreated with an antioxidant, 10 mM NAC (Sigma) for 30 min before the hydrogen peroxide treatment

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Substrates were clustered by activity index across strains using pvclust

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Next the pTwist-Lenti-HSPA1L-mRuby2 plasmid was packaged into lentivirus by co-transfection of psPAX2 (Gift from Didier Trono, Addgene #12259), and pMD2-G (Gift from Didier Trono, Addgene #12260) at equimolar concentrations into HEK293T cells

Conclusion Glutathione is a vital antioxidant that plays a significant role in maintaining our health and well-being