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Based on the molecular weight, isolated LPS can potentially cross the epithelium through the paracellular pathway as has been suggested in some studies ( Contrary to the widely held assumption that whole bacteria can cross the intestinal epithelium via the paracellular pathway, basic research strongly supports that endocytosis is the mechanism used for bacteria to translocate to the lamina propria ( Despite the increased LPS translocation into the blood described in numerous pathologies, it is not clear whether blood endotoxin levels are the result of LPS from the lumen or whether LPS is released by whole bacteria translocated in the lamina propria by the wall breakdown from effective host defense mechanisms and by autolysis ( Methods to Assess Intestinal Permeability Ex vivo Assessment of Intestinal Permeability With the Ussing Chambers Technique In 1951, the Danish scientist Hans Ussing performed for the first time electrophysiology in the frog skin ( Transepithelial Electrical Resistance and Paracellular Permeability Currently, there are different commercially available Ussing chamber equipments that are designed to accommodate multiple epithelial sources such as cell cultures grown in membranes, animal tissue and human tissue from resection specimens or endoscopic biopsies