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Hematoxylin-eosin staining was performed to observe hepatic histology

doi: 10.1084/jem.20110251 82 HeSLiangYShaoFWangX

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Kidneys were then excised and enzymatically digested in a solution containing 213 g/ml Liberase (Roche Diagnostics, Mannheim, Germany), 100 g/ml DNase I (Roche), and 40 g/ml collagenase IV (Gibco, Gaithersburg, MD) at 37 C for 20 min

Duration of treatment varies based on condition severity and individual response

Figure 3a illustrates that these genes were upregulated at 6 h but minimally affected at 3 h