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Complementary DNA (cDNA) was made by reverse transcription of RNA (2 g) using Moloney murine leukemia virus reverse transcriptase (Promega, Madison, MI, USA) and labeled with Biotin-16-2-deoxy-uridine-5-triphosphate (Roche Diagnostics, Indianapolis, IN, USA)

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21 The aforementioned bacteria are recognized for having probiotic activity, which points to the importance of the relationship between vitamin B 12 and the gut microbiota, because probiotics are defined as living microorganisms that provide benefits to the hosts health when administered in adequate doses