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Meanwhile, natural and synthetic antioxidants, such as chlorogenic acid, have a certain mitigation effect on the formation of furan induced by heated ascorbic acid, but the mitigation effect may decrease with the increase in heating time [72]

Western blotting Cell samples and liver tissues (1020 mg) were lysed, and equal amounts of protein were denatured after detection of the protein concentration using a BCA protein assay kit (Thermo Scientific)

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