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For labelling, the fixed cells were thawed and washed for 5 min with 1 ml of 1% BSA (Sigma-Aldrich, A7906) in PBS

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In general transfusion should aim to keep patients haemoglobin levels at between 9-10.5g/dl before transfusion

One common feature to both animal models of SOD1 ALS and human disease is the deposition of aggregate SOD1 protein in neuronal and glial inclusions (Philips & Rothstein, 2015;Stieber et al., 2000