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Results GSH depletion induces cell death in RPE cells To evaluate the effect of GSH depletion on the RPE cells, we used three different approaches: depletion of Cys 2 from cell culture media, treatment with buthionine sulphoximine (BSO, 1000 M) to block de novo synthesis of GSH, or treatment with erastin (10 M), an inhibitor of system x c 1

Hypoxia Induces Production of L-2-Hydroxyglutarate

Oral glutathione supplementation drastically reduces Helicobacter -induced gastric pathologies

Furthermore, mitochondrial oxidative stress has been demonstrated to induce ferroptosis via the NRF2-ARE pathway, thereby emphasizing the multifaceted role of ROS in disease mechanisms

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