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Neurotoxicity was almost completely prevented by the presence of either NMDA receptor antagonists, MK-801 and D(-)-2-amino-5-phosphonopentanoic acid (D-AP5), or mGluR antagonist, (S)-4-carboxyphenylglycine (S-4CPG)

For glutathionylated preparations made at pH 6.8 the M pro was incubated for 15 min at 37C in 50 mM Tris, pH 7.5, 2 mM EDTA, and 100 mM NaCl containing 100 ug/ml of cm-BSA, Grx (88-350 nM), 0.1 mM GSH or 0.1 mM GSH with 88-350 nM Grx in a total volume of 10 L

Hypoxia increases the deoxycytidine triphosphate (dCTP) level through the pentose phosphate pathway (PPP) via glucose metabolism and results in resistance to gemcitabine, a dCTP analog 87

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Stress boosts oxidative stress, which depletes glutathione and impairs antioxidant defenses