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The methodologies utilized in this research included employing 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), lactate dehydrogenase (LDH), and Calcein-AM assays to evaluate the extent of neural damage

It's whether the research question justifies the added complexity

PMID: 26371405

Gradual Introduction: Start with lower concentrations (e.g., 0.05% to 1%) and less frequent application (e.g., every other day) to allow the skin to acclimate, especially for sensitive skin types

Early Warning Signs The early signs of B12 deficiency are often subtle and difficult to recognize