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The construction of the bsh 1 mutagenesis vector was achieved through the following methodology: Strain AR113 served as the template for PCR amplification, employing the primer sets BSH1up-F and BSH1up-R, BSH1down-F and BSH1down-R, and BSH1gRNA-F and BSH1gRNA-R to generate BSH1up, BSH1down, and BSH1gRNA, respectively (Table S1)
and, as might have been expected, he found Columbus guilty of every charge made against him
