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For the detection of transgenes, DNA was subjected to qPCR, and SYBR Premix ExTaqII (Takara Bio, Shiga, Japan) was used for the detection
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Cultured hepatocytes exhibit basal GCLC phosphorylation, which increased when treated with DBcAMP or phenylephrine, suggesting GCLC may be under a basal inhibitory tone [128]
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