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On day 6 of differentiation, the medium was changed to glucose/phenol red/glutamine/pyruvate-free DMEM (Thermo Fisher Scientific) supplemented with 0.5% FCS, 4.5 g l 1 glucose, 4 mM glutamine, 0.5 mM pyruvate and 5 g ml 1 insulin

Packer L, Witt EH, Tritschler HJ (1995) Alpha-lipoic acid as a biological antioxidant
Automated high-performance liquid chromatographic method for the determination of guanidinoacetic acid in dried blood spots: a tool for early diagnosis of guanidinoacetate methyltransferase deficiency

The final sample comprised 331 patients (160 males and 171 females), aged 6.5 to 52 years (M = 18.19, SD = 8.72)

Short, daily dips in parasympathetic activity reduce sympathetic overdrive and cortisol pulses that impact the hypothalamic-pituitary-gonadal axis
