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8-12ml/g In the peptide cutting reagent, the concentration of the resin is 8-12ml/g C 18 C 8 According to the present invention, the method for purifying the crude product through C18 or C8 chromatographic column comprises the steps: A0.199.9B0.199.91-150ml/min215285nm There are two mobile phases: A: 0.1% trifluoroacetic acid plus 99.9% water

doi: 10.1161/ATVBAHA.124.321201

Kapahi, P

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k cat and K m values could not be obtained due to the low activity of the mutant enzymes

The activity of liver and bone ALPs in the serum has been extensively used in routine diagnosis