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Development of a biomimetic enzyme-linked immunosorbent assay method for the determination of estrone in environmental water using novel molecularly imprinted films of controlled thickness as artificial antibodies

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Groundwater is often cheaper, more convenient and less vulnerable to pollution than surface water

The procedure is based on the reduction of Ellmans reagent by -SH groups of GSH to form 2-nitro-s-mercaptobenzoic acid, the nitromercaptobenzoic acid anion has an intense yellow color which can be determined spectrophotometrically