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European Journal of Medical Genetics, 58(1), 110

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To evaluate the use of this enzyme in manipulating compartment-specific GSH pools, we transduced HEK 293T cells with either untargeted or mitochondria-targeted, human codon-optimized GshF , henceforth referred to respectively as GshF and mito-GshF , and confirmed their subcellular localization by cell fractionation (Fig

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The radioactive proteins synthesized are separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and detected by radioautography

Proteinuria can, for example, result from nonxenobiotic tubular injury and from glomerular disease, and is also associated with diabetes, cardiovascular disease, and inflammation