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Microcystin-LR does not alter cell survival and intracellular signaling in human bronchial epithelial cells

[DOI] [PMC free article] [PubMed] [Google Scholar] 252.Parlak O., Keene S.T., Marais A., Curto V.F., Salleo A

Coggins J

Moreover, mTOR kinase activity may be suppressed by the activation of AMPK itself 11

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The design principle: Standard (often fails): His6 - TEV site - Target protein Better: His6 - TEV site - (GGS)2 - Target protein Even better: His6 - (GGS)2 - TEV site - (GGS)2 - Target protein Failure 2: Non-Specific Cleavage (The Protease Damages Your Protein) The problem: Proteases aren't perfectly specific