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Western blotting For whole-cell protein extraction, cell pellets were lysed in cold RIPA buffer (50 mM TrisHCL pH 7.4, 5 mM EDTA, 250 mM NaCl, 50 mM NaF, 0.1 mM Na 3 VO 4 , 0.1% Triton X-100), supplemented before use with cOmplete Protease Inhibitor Cocktail (Roche) and 5 mM sodium butyrate

Rotate injection sites within this general area to prevent localized irritation or scar tissue formation

25 This process not only clears necrotic debris but also advances liver repair, opening new avenues for therapeutic strategies in liver repair and regeneration