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Following nickel purification (Figure 4A, lanes 2 and 3), the enriched fraction showed a high degree of purity and appropriate size as a monomer in absence of glutathione (lane 2) or as a dimer in the presence of glutathione (lane 3)

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Further purification and characterization

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Then, the changes in O 2 concentration were determined in 2 min by a dissolved oxygen analyzer

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Yamamoto, S

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