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Lysate was clarified by centrifugation (20,000 g , 10 minutes, 4 C)

Similarly, subcutaneous administration of progesterone prevented mechanical and thermal allodynia in male rats following SCI rats, and decreased levels of the pro-inflammatory cytokines IL-1, IL-6, and TNF in the spinal cord dorsal horn, supporting the notion that progesterone has a capability of suppressing reactive gliosis following CNS injury [373]

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Regioselective hydroxylation of C(12)-C(15) fatty acids with fluorinated substituents by cytochrome P450 BM3