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Toonen RF, Wierda K, Sons MS, de Wit H, Cornelisse LN, Brussaard A, et al

Columns were then washed with no-detergent wash buffer (25 mM HEPES pH 7.4, 150 mM KOAc, 5 mM NaCl, 2 mM Mg(OAc) 2 , 5% glycerol) and proteins were eluted by two sequential additions of 4 ml elution buffer (25 mM HEPES pH 7.4, 25 mM Tris pH 8, 150 mM KOAc, 5 mM NaCl, 2 mM Mg(OAc) 2 ) containing either 10 mM reduced glutathione for the glutathione Sepharose pulldown or 20 mM biotin for the StreptactinXT pulldown

A.El-ZaidyM.AliS

(2024) 5:e520

Michikoshi H, Nakamura T, Sakai K, Suzuki Y, Adachi E, Matsugo S, et al

The basis of this decontamination method is to increase the rate at which the water in the body is replaced with new water