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Carr AC, Spencer E, Das A, Meijer N, Lauren C, Macpherson S, et al

doi: 10.1016/s0009-2797(97)00155-5

The supernatant was discarded, and the bacterial pellet was washed twice with PBS to remove any unincorporated probes

A., Selbach O

PMID 8312546

GSSG-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour
