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The loading was stopped with a Ringer-like (control) solution containing (mmol/l): 150 NaCl, 6 CsCl, 1 MgCl 2 , 10 glucose, 10 HEPES, and 1.5 CaCl 2 at pH 7.4 and 317 mOsmol/l [17]
Loss of Tsc2 in radial glia models the brain pathology of tuberous sclerosis complex in the mouse
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Coverslips were then blocked with 3% BSA/PBS and incubated in anti-BrdU primary antibody (1:500) followed by incubation in FITC anti-Rat secondary antibody (1:1,000)
