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The pH-dependence of the EgtE UV-vis spectra The universal buffer containing 25 mM NaOAc, 25 mM MES, 25 mM glycine, 75 mM Tris was prepared as 6.0, 7.0, 8.0, 9.0, 10.0

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In some cases, especially for individuals with digestive issues or malabsorption conditions, the efficiency of oral supplementation can be severely compromised, leading to nutrient deficiencies despite adequate intake

The mobile phases used in the experiments were buffer A (0.1% formic acid in water) and buffer B (0.1% formic acid in water acetonitrile)

[DOI] [PMC free article] [PubMed] [Google Scholar] Kulju KS, Lehman JM

Marasas, and Leon Sokoloff