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To terminate the reactions, an SDS loading buffer was introduced, and subsequent analyses were performed by transferring the samples to PVDF membranes for probing with either an anti-phospho-S32-RIPK1 or an anti-phospho-S166-RIPK1 specific antibody

10.1111/j.1365-2222.2008.02958.x 7

Intravenous B-12 carries significantly higher anaphylaxis risk and is not standard practice in most clinics

doi: 10.1111/j.1440-1819.2009.02015.x

First- and second-strand cDNA synthesis, end-filling with Klenow fragment, and dA-tailing were performed per manufacturers instructions