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The cells were treated with these cocktails for 3 days, with variations in CHIR concentration and the presence of Ly or PD to optimize DE induction efficiency [1]

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At any point in this process, the -arrestins may also recruit other proteinssuch as the non-receptor tyrosine kinase (nRTK), c-SRCwhich may activate ERK1/2, or other mitogen-activated protein kinase (MAPK) signaling through, for example, phosphorylation of the small GTPase, Ras, or recruit the proteins of the ERK cascade directly (i.e., Raf-1, MEK, ERK-1/2) at which point signaling is initiated due to their close proximity to one another