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Iliceto M, et al

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[DOI] [PMC free article] [PubMed] [Google Scholar] Kalea, A

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To study protein-protein interactions in vitro, DNA encoding mature SLP L or SLP H of R7404 was amplified using Q5 (NEB) PCR and cloned into pET28a using Nco I- Xho I restriction cloning, in frame with a C-terminal 6xHis-tag