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What Happens If You Dont Get Enough Vitamin B12

j , Medium of WT and Smurf1 KO MEFs were quantified by the absorbance at 570 nm using microplate reader, and the standard curve of lactate concentration was calculated from samples containing certain concentrations of lactate (n = 3 independent experiments)

Following this, the protein lysates were denatured in a metal bath at 95 C and subjected to SDS-polyacrylamide gel electrophoresis (SDS-PAGE) for separation

Always check product labels, as fortification levels vary considerably between brands