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Description
An additional group of age-matched, unoperated animals served as unoperated controls ( n = 4, all female)
The following antibodies were used: rabbit anti-ionized calcium binding adaptor molecule 1 (Iba1) polyclonal antibody (1:100, Wako Chemicals), mouse anti-NeuN monoclonal antibody (1:1000, EMD Millipore Bioscience Research Reagents), rabbit anti-glial fibrillary acidic protein (GFAP) polyclonal antibody (1:1000, Dako, RRID: AB_10013382), mouse anti-PECAM1 (CD31) monoclonal antibody (1:250, EMD Millipore), and goat anti-COX2 (M-19) polyclonal antibody (1:5000, Santa Cruz)
33 Common neurological symptoms include myelopathy, neuropathy, and, less frequently, optic nerve atrophy
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The diet-induced release of immune mediators into the systemic circulation is referred to as metabolic endotoxemia, which can lead to immune activation in different organs, including the brain 102
doi: 10.1093/brain/awt224
