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A431 epidermoid carcinoma cells (ATCC, CRL-1555) were grown in DMEM (Gibco, #41966-029) with 10% FBS and 1% PenStrep

9) DHEA (dehyrdoepiandosterone) - DHEA is a weak prohormone produced by the adrenal gland

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Clean the skin at the injection site with an alcohol swab and let it dry

[51] Diagnosis [edit] According to one review: "At present, no 'gold standard' test exists for the diagnosis of vitamin B 12 deficiency and as a consequence the diagnosis requires consideration of both the clinical state of the patient and the results of investigations." [52] The vitamin deficiency is typically suspected when a routine complete blood count shows anemia with an elevated mean corpuscular volume (MCV)

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