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Nthy-ori3-1 human thyroid normal cells were purchased from Zhaoqing Sea Star Biotechnology Co., LTD., and then cultured in RPMI-1640 supplemented with 10% fetal bovine serum (Corning), 10,000 U/mL penicillin, as well as 10 mg/mL streptomycin (Corning)

The column was maintained at 35C