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Myth: Its okay to use the same needle to draw and inject. Fact: While technically possible, many people use a larger needle to draw the water and a much smaller, thinner needle for the actual injection to maximize comfort

Understanding proper reconstitution technique prevents damage to the peptide during preparation

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Each cuvette contained 50 mM Tris-HCl (pH 7.4) buffer, 75 mM sodium chloride, 0.01 M sodium cyanide, 0.5 mM MTT, 0.2 mM PMS, and 0.2 ml of cell extract