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Preclinical Research Notice: The scientific references cited in this article and on the ProHealth product page describe preclinical research conducted primarily in animal models and laboratory settings

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The Gibbs free energy for sub-problem is given bywhere are the standard Gibbs free energies of reaction at 25C for the metabolic reactions associated with sub-problem , are mole fractions, which are calculated from the fluxes, are chemical potentials, is a component index, and is the number of components associated with a sub-problem in the network