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The samples were then incubated with the equilibration buffer provided in the TUNEL assay kit at room temperature for 10 min, followed by incubation with the reaction mixture containing terminal deoxynucleotidyl transferase and labeled dUTP

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The concentrated solution was dialyzed with deionized water for 72 h, and then lyophilized to obtain D-P (yield: 90%)

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It does this by taking the "load" off the glutathione

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For LCMS analysis, metabolite extracts were analysed by high-resolution mass spectrometry with an Orbitrap Exploris 240 (Thermo) coupled to a Vanquish Flex liquid chromatography system (Thermo)

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