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Besselink MGH, van Santvoort HC, Buskens E, et al

MiR-122 inhibition leads to decreased plasma cholesterol and triglyceride levels associated with altered lipids biosynthesis and increased FAO

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The cell pellets were resuspended in 800 L of ice-cold 80% (v/v) methanol, mixed with 0.35 g of 0.2 mm zirconia-silica beads (BioSpec Products Inc., Bartlesville, OK, United States) in 1.5 mL microtubes, and cooled on ice