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Furthermore, because some NAPQI is created regardless of the function of the other 2 pathways, failure in the glutathione-dependent pathway is expected to result in the accumulation of NAPQI and subsequent toxicity, even if the other 2 pathways are functional

Intravenous administration is not advised due to potential adverse effects

Others have linked low glutathione levels to lack of sleep in animals and in people with certain sleep disorders

This is by design

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The apparent Km (310M) for the NADPH cofactor is similar for both isozymes