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Cooper, M

The oral intake of specific bovine-derived bioactive collagen peptides has a stimulatory effect on dermal matrix synthesis and improves various clinical skin parameters

Severe deficiency or neurological symptoms require injectable b12 for faster repletion

Although rare, adverse effects of high-dose vitamin B12 have been reported, including allergic reactions such as fever, rash, itching, hot flushes, dizziness, and nausea, across all cobalamin forms and administration routes ( Intramuscular (IM) injection is the most invasive and costly administration method, requiring trained personnel and clinical visits

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Fixative was then removed and neutralized twice with 100 mM Tris Buffered Saline in MilliQ water and then washed once with 0.1% Tween-20 in PBS