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Description
Because Cys20 oxidizes naturally as well as artificially, the C20S mutant was used for structural determination of the complexes

LHCGR engagement activates Gs-coupled adenylyl cyclase, driving cAMP accumulation and PKA-mediated phosphorylation of downstream steroidogenic targets including CYP11A1, CYP17A1, and 3-HSD

SiRNA transfection and lentivirus transduction Specific siRNA targeting AMPK1 (Ribobio, Guangzhou, China) were transfected into the indicated cells using Lipofectamine 2000 (Invitrogen, CA, USA) according to the manufacturers instructions

Conclusion The E-P-OS network framework offers a novel perspective for understanding the persistent growth of uterine fibroids and their recurrence following conventional treatments

A stair lift can help reduce surgery recovery time by preventing additional injury from walking up and downstairs

Statistical analysis Graphs and statistical analysis were conducted using R (version 3.2.5) and Adobe illustrator CC 2015
